Irradiation and fusion gene transfer
Identifieur interne : 000934 ( Main/Exploration ); précédent : 000933; suivant : 000935Irradiation and fusion gene transfer
Auteurs : Michael A. Walter [Canada] ; Peter N. Goodfellow [Royaume-Uni]Source :
- Molecular Biotechnology [ 1073-6085 ] ; 1995-04-01.
English descriptors
- KwdEn :
- Teeft :
- Bayesian methods, Boehnke, Brcal region, Cell fusion, Cell genet, Cell hybrids, Cell line, Cell lines, Chromosomal, Chromosome, Clone, Critical factors, Disease loci, Distal region, Donor cell, Donor cells, Dos, Entire genome, Fragment, Fusion gene transfer, Gene, Genet, Genetic linkage analysis, Genome, Genomics, Goodfellow, Growth medium, High doses, High retention frequencies, Human chromosomal region, Human chromosome, Human chromosome fragments, Human chromosomes, Human fragments, Human genes, Human genome, Human marker, Human material, Hybrid, Hybrid cells, Hybrid clones, Hybrid lines, Ifgt, Ifgt data, Incontinentia pigmenti, Irradiation hybrids, Irradiation step, Krad, Lethal dose, Locus, Locus order, Mammalian cells, Mapping, Mapping panel, Marker, Marker frequency, Marker retention, Marker retention frequencies, Maximum likelihood methods, Microtiter plates, Molecular methods, Physical mapping techniques, Physical maps, Polyethylene glycol, Radiation dosage, Radiation dose, Radiation doses, Radiation hybrids, Rapid isolation, Recipient cell line, Recipient cell lines, Repetitive sequence polymerase chain reaction, Retention frequency, Rodent portion, Room temperature, Same cell lines, Selectable marker, Selectable markers, Simple screen, Single panel, Somat, Somatic, Somatic cell, Somatic cell hybrids, Somatic cells, Statistical analysis, Statistical methods, Such hybrids, Translocation breakpoints, Trends genet, True hybrids, Useful addition.
Abstract
Abstract: Irradiation and fusion gene transfer (IFGT) is a technique that spans the gap between the limitations of molecular methods and somatic-cell genetics, allowing the separation of DNA fragments between 0.25 and 30 Mb in size. In conjunction with genetic linkage analysis and physical mapping techniques, IFGT provides a very useful addition to methods for cloning disease loci, and mapping chromosomes and entire genomes.
Url:
DOI: 10.1007/BF02789107
Affiliations:
Links toward previous steps (curation, corpus...)
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Le document en format XML
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Walter</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Ophthalmology, University of Alberta, Edmonton, AB.</wicri:regionArea><wicri:noRegion>AB.</wicri:noRegion></affiliation></author><author><name sortKey="Goodfellow, Peter N" sort="Goodfellow, Peter N" uniqKey="Goodfellow P" first="Peter N." last="Goodfellow">Peter N. Goodfellow</name><affiliation wicri:level="2"><country>Royaume-Uni</country><placeName><region type="country">Angleterre</region></placeName><wicri:cityArea>Department of Genetics, University of Cambridge, Cambridge</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Biotechnology</title><title level="j" type="abbrev">Mol Biotechnol</title><idno type="ISSN">1073-6085</idno><idno type="eISSN">1559-0305</idno><imprint><publisher>Humana Press</publisher><pubPlace>Totowa</pubPlace><date type="published" when="1995-04-01">1995-04-01</date><biblScope unit="volume">3</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="117">117</biblScope><biblScope unit="page" to="128">128</biblScope></imprint><idno type="ISSN">1073-6085</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1073-6085</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Irradiation and fusion gene transfer (IFGT)</term><term>physical mapping</term><term>radiation hybrids (RH)</term><term>somatic cell hybrids</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Bayesian methods</term><term>Boehnke</term><term>Brcal region</term><term>Cell fusion</term><term>Cell genet</term><term>Cell hybrids</term><term>Cell line</term><term>Cell lines</term><term>Chromosomal</term><term>Chromosome</term><term>Clone</term><term>Critical factors</term><term>Disease loci</term><term>Distal region</term><term>Donor cell</term><term>Donor cells</term><term>Dos</term><term>Entire genome</term><term>Fragment</term><term>Fusion gene transfer</term><term>Gene</term><term>Genet</term><term>Genetic linkage analysis</term><term>Genome</term><term>Genomics</term><term>Goodfellow</term><term>Growth medium</term><term>High doses</term><term>High retention frequencies</term><term>Human chromosomal region</term><term>Human chromosome</term><term>Human chromosome fragments</term><term>Human chromosomes</term><term>Human fragments</term><term>Human genes</term><term>Human genome</term><term>Human marker</term><term>Human material</term><term>Hybrid</term><term>Hybrid cells</term><term>Hybrid clones</term><term>Hybrid lines</term><term>Ifgt</term><term>Ifgt data</term><term>Incontinentia pigmenti</term><term>Irradiation hybrids</term><term>Irradiation step</term><term>Krad</term><term>Lethal dose</term><term>Locus</term><term>Locus order</term><term>Mammalian cells</term><term>Mapping</term><term>Mapping panel</term><term>Marker</term><term>Marker frequency</term><term>Marker retention</term><term>Marker retention frequencies</term><term>Maximum likelihood methods</term><term>Microtiter plates</term><term>Molecular methods</term><term>Physical mapping techniques</term><term>Physical maps</term><term>Polyethylene glycol</term><term>Radiation dosage</term><term>Radiation dose</term><term>Radiation doses</term><term>Radiation hybrids</term><term>Rapid isolation</term><term>Recipient cell line</term><term>Recipient cell lines</term><term>Repetitive sequence polymerase chain reaction</term><term>Retention frequency</term><term>Rodent portion</term><term>Room temperature</term><term>Same cell lines</term><term>Selectable marker</term><term>Selectable markers</term><term>Simple screen</term><term>Single panel</term><term>Somat</term><term>Somatic</term><term>Somatic cell</term><term>Somatic cell hybrids</term><term>Somatic cells</term><term>Statistical analysis</term><term>Statistical methods</term><term>Such hybrids</term><term>Translocation breakpoints</term><term>Trends genet</term><term>True hybrids</term><term>Useful addition</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Irradiation and fusion gene transfer (IFGT) is a technique that spans the gap between the limitations of molecular methods and somatic-cell genetics, allowing the separation of DNA fragments between 0.25 and 30 Mb in size. 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